|
Treatment
|
Cell no. as % Control (SD)
|
Putrescine (SD)
|
Spermidine (SD)
|
Spermine (SD)
|
|---|
|
24 hour
| | | | |
|
Control
|
100.0 (0.0)
|
0.48 (0.31)
|
1.62 (0.55)
|
2.10 (0.98)
|
|
I3C 100
|
89.1 (19.8)
|
0.31 (0.09)
|
1.79 (0.51)
|
2.25 (1.21)
|
|
I3C 175
|
87.9 (29.2)
|
0.23 (0.06)*
|
1.32 (0.36)
|
1.54 (0.19)
|
|
I3C 250
|
77.4 (32.7)
|
0.19 (0.04)*
|
2.17 (0.98)
|
2.73 (1.86)
|
|
I3C 500
|
54.2 (26.6)
|
0.14 (0.07)*
|
1.57 (0.60)
|
2.74 (1.82)
|
|
C + P20
|
96.9 (23.5)
|
0.64 (0.26)
|
2.17 (1.06)
|
2.41 (1.53)
|
|
C + P50
|
90.0 (42.1)
|
0.72 (0.27)*
|
2.34 (1.48)
|
2.59 (1.93)
|
|
I + P20
|
76.2 (26.3)
|
0.69 (0.29)
|
2.93 (1.57)*
|
3.06 (1.99)
|
|
I + P50
|
76.1 (35.0)
|
0.54 (0.16)
|
1.86 (0.49)
|
1.94 (0.65)
|
|
96 hour
| | | | |
|
Control
|
100.0 (0.0)
|
0.08 (0.04)
|
0.79 (0.30)
|
1.25 (0.38)
|
|
I3C 100
|
98.7 (8.5)
|
0.13 (0.09)
|
1.11 (0.40)
|
1.87 (0.47)
|
|
I3C 175
|
58.9 (3.8)
|
0.13 (0.10)
|
0.98 (0.29)
|
2.18 (0.79)*
|
|
I3C 250
|
24.9 (6.1)
|
0.15 (0.11)
|
0.77 (0.36)
|
2.03 (1.17)*
|
|
I3C 500
|
13.9 (6.0)
|
nd
|
0.62 (0.32)
|
1.34 (0.45)
|
|
C + P20
|
96.6 (12.4)
|
0.13 (0.03)
|
0.84 (0.15)
|
1.15 (0.12)
|
|
C + P50
|
79.2 (14.3)
|
0.32 (0.18)*
|
1.47 (0.15)*
|
1.98 (0.34)
|
|
I + P20
|
28.1 (22.6)
|
0.17 (0.09)
|
1.25 (0.23)*
|
2.42 (0.21)*
|
|
I + P50
|
17.9 (2.9)
|
0.11 (0.02)
|
0.80 (0.21)
|
1.54 (0.48)
|
|
168 hour
| | | | |
|
Control
|
100.0 (0.0)
|
0.04 (0.02)
|
0.46 (0.07)
|
0.76 (0.17)
|
|
I3C 100
|
104.0 (9.9)
|
0.03 (0.01)
|
0.57 (0.25)
|
1.22 (0.32)
|
|
I3C 175
|
57.1 (9.5)
|
0.05 (0.03)
|
0.62 (0.28)
|
1.99 (0.96)*
|
|
I3C 250
|
31.1 (20.2)
|
0.07 (0.01)
|
0.87 (0.54)
|
2.93 (0.72)*
|
|
I3C 500
|
4.5 (5.2)
|
nd
|
nd
|
nd
|
|
C + P20
|
107.2 (4.8)
|
0.03 (0.01)
|
0.48 (0.04)
|
0.83 (0.15)
|
|
C + P50
|
104.5 (10.1)
|
0.06 (0.04)
|
0.67 (0.10)
|
1.08 (0.19)
|
|
I + P20
|
6.0 (5.5)
|
nd
|
0.91 (0.55)
|
3.11 (1.59)*
|
|
I + P50
|
3.0 (0.7)
|
nd
|
0.90 (0.56)
|
2.26 (1.42)*
|
- Cells were treated with I3C alone or in combination with putrescine, and intracellular polyamines determined as described in the Methods Section. Data shown for cell numbers are expressed as a % Control, and polyamine levels are nmol/106 cells. All values are means with SD in brackets. nd = values not determined. * indicates a value that is significantly different to the control value at that time point.
