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Table 1 Effect of I3C alone and in combination with putrescine on intracellular polyamine levels at 24, 96 and 168 hours.

From: Growth-inhibitory effects of the chemopreventive agent indole-3-carbinol are increased in combination with the polyamine putrescine in the SW480 colon tumour cell line

Treatment

Cell no. as % Control (SD)

Putrescine (SD)

Spermidine (SD)

Spermine (SD)

24 hour

    

Control

100.0 (0.0)

0.48 (0.31)

1.62 (0.55)

2.10 (0.98)

I3C 100

89.1 (19.8)

0.31 (0.09)

1.79 (0.51)

2.25 (1.21)

I3C 175

87.9 (29.2)

0.23 (0.06)*

1.32 (0.36)

1.54 (0.19)

I3C 250

77.4 (32.7)

0.19 (0.04)*

2.17 (0.98)

2.73 (1.86)

I3C 500

54.2 (26.6)

0.14 (0.07)*

1.57 (0.60)

2.74 (1.82)

C + P20

96.9 (23.5)

0.64 (0.26)

2.17 (1.06)

2.41 (1.53)

C + P50

90.0 (42.1)

0.72 (0.27)*

2.34 (1.48)

2.59 (1.93)

I + P20

76.2 (26.3)

0.69 (0.29)

2.93 (1.57)*

3.06 (1.99)

I + P50

76.1 (35.0)

0.54 (0.16)

1.86 (0.49)

1.94 (0.65)

96 hour

    

Control

100.0 (0.0)

0.08 (0.04)

0.79 (0.30)

1.25 (0.38)

I3C 100

98.7 (8.5)

0.13 (0.09)

1.11 (0.40)

1.87 (0.47)

I3C 175

58.9 (3.8)

0.13 (0.10)

0.98 (0.29)

2.18 (0.79)*

I3C 250

24.9 (6.1)

0.15 (0.11)

0.77 (0.36)

2.03 (1.17)*

I3C 500

13.9 (6.0)

nd

0.62 (0.32)

1.34 (0.45)

C + P20

96.6 (12.4)

0.13 (0.03)

0.84 (0.15)

1.15 (0.12)

C + P50

79.2 (14.3)

0.32 (0.18)*

1.47 (0.15)*

1.98 (0.34)

I + P20

28.1 (22.6)

0.17 (0.09)

1.25 (0.23)*

2.42 (0.21)*

I + P50

17.9 (2.9)

0.11 (0.02)

0.80 (0.21)

1.54 (0.48)

168 hour

    

Control

100.0 (0.0)

0.04 (0.02)

0.46 (0.07)

0.76 (0.17)

I3C 100

104.0 (9.9)

0.03 (0.01)

0.57 (0.25)

1.22 (0.32)

I3C 175

57.1 (9.5)

0.05 (0.03)

0.62 (0.28)

1.99 (0.96)*

I3C 250

31.1 (20.2)

0.07 (0.01)

0.87 (0.54)

2.93 (0.72)*

I3C 500

4.5 (5.2)

nd

nd

nd

C + P20

107.2 (4.8)

0.03 (0.01)

0.48 (0.04)

0.83 (0.15)

C + P50

104.5 (10.1)

0.06 (0.04)

0.67 (0.10)

1.08 (0.19)

I + P20

6.0 (5.5)

nd

0.91 (0.55)

3.11 (1.59)*

I + P50

3.0 (0.7)

nd

0.90 (0.56)

2.26 (1.42)*

  1. Cells were treated with I3C alone or in combination with putrescine, and intracellular polyamines determined as described in the Methods Section. Data shown for cell numbers are expressed as a % Control, and polyamine levels are nmol/106 cells. All values are means with SD in brackets. nd = values not determined. * indicates a value that is significantly different to the control value at that time point.