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Table 1 Effect of I3C alone and in combination with putrescine on intracellular polyamine levels at 24, 96 and 168 hours.

From: Growth-inhibitory effects of the chemopreventive agent indole-3-carbinol are increased in combination with the polyamine putrescine in the SW480 colon tumour cell line

Treatment Cell no. as % Control (SD) Putrescine (SD) Spermidine (SD) Spermine (SD)
24 hour     
Control 100.0 (0.0) 0.48 (0.31) 1.62 (0.55) 2.10 (0.98)
I3C 100 89.1 (19.8) 0.31 (0.09) 1.79 (0.51) 2.25 (1.21)
I3C 175 87.9 (29.2) 0.23 (0.06)* 1.32 (0.36) 1.54 (0.19)
I3C 250 77.4 (32.7) 0.19 (0.04)* 2.17 (0.98) 2.73 (1.86)
I3C 500 54.2 (26.6) 0.14 (0.07)* 1.57 (0.60) 2.74 (1.82)
C + P20 96.9 (23.5) 0.64 (0.26) 2.17 (1.06) 2.41 (1.53)
C + P50 90.0 (42.1) 0.72 (0.27)* 2.34 (1.48) 2.59 (1.93)
I + P20 76.2 (26.3) 0.69 (0.29) 2.93 (1.57)* 3.06 (1.99)
I + P50 76.1 (35.0) 0.54 (0.16) 1.86 (0.49) 1.94 (0.65)
96 hour     
Control 100.0 (0.0) 0.08 (0.04) 0.79 (0.30) 1.25 (0.38)
I3C 100 98.7 (8.5) 0.13 (0.09) 1.11 (0.40) 1.87 (0.47)
I3C 175 58.9 (3.8) 0.13 (0.10) 0.98 (0.29) 2.18 (0.79)*
I3C 250 24.9 (6.1) 0.15 (0.11) 0.77 (0.36) 2.03 (1.17)*
I3C 500 13.9 (6.0) nd 0.62 (0.32) 1.34 (0.45)
C + P20 96.6 (12.4) 0.13 (0.03) 0.84 (0.15) 1.15 (0.12)
C + P50 79.2 (14.3) 0.32 (0.18)* 1.47 (0.15)* 1.98 (0.34)
I + P20 28.1 (22.6) 0.17 (0.09) 1.25 (0.23)* 2.42 (0.21)*
I + P50 17.9 (2.9) 0.11 (0.02) 0.80 (0.21) 1.54 (0.48)
168 hour     
Control 100.0 (0.0) 0.04 (0.02) 0.46 (0.07) 0.76 (0.17)
I3C 100 104.0 (9.9) 0.03 (0.01) 0.57 (0.25) 1.22 (0.32)
I3C 175 57.1 (9.5) 0.05 (0.03) 0.62 (0.28) 1.99 (0.96)*
I3C 250 31.1 (20.2) 0.07 (0.01) 0.87 (0.54) 2.93 (0.72)*
I3C 500 4.5 (5.2) nd nd nd
C + P20 107.2 (4.8) 0.03 (0.01) 0.48 (0.04) 0.83 (0.15)
C + P50 104.5 (10.1) 0.06 (0.04) 0.67 (0.10) 1.08 (0.19)
I + P20 6.0 (5.5) nd 0.91 (0.55) 3.11 (1.59)*
I + P50 3.0 (0.7) nd 0.90 (0.56) 2.26 (1.42)*
  1. Cells were treated with I3C alone or in combination with putrescine, and intracellular polyamines determined as described in the Methods Section. Data shown for cell numbers are expressed as a % Control, and polyamine levels are nmol/106 cells. All values are means with SD in brackets. nd = values not determined. * indicates a value that is significantly different to the control value at that time point.
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