Figure 3From: Growth-inhibitory effects of the chemopreventive agent indole-3-carbinol are increased in combination with the polyamine putrescine in the SW480 colon tumour cell line Effect of putrescine supplementation on cell growth inhibition by I3C and DFMO. Cells were treated with I3C (SW480 175 μM; HT29 250 μM; HCEC 180 μM) or DFMO (SW480 50 μM; HT29 and HCEC 125 μM) or DMSO (control) and cultured in the presence or absence of putrescine (5–500 μM) for 7 days and then counted as described in Materials and Methods. Results are mean ± SD (n = 4), a indicates bars which are significantly different from control (DMSO without putrescine) group, b indicates significant difference from the I3C alone treatment, and c indicates significant difference from the DFMO alone treatment (P < 0.05).Back to article page