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Figure 1 | BMC Cancer

Figure 1

From: Increased expression of AP2 and Sp1 transcription factors in human thyroid tumors: a role in NIS expression regulation?

Figure 1

Competition for binding between thyroid nuclear proteins and AP1 and Sp1 consensus oligonucleotides. 212 bp NIS probe was 5' end-labeled and used in gel retardation assays with 10 μg of thyroid tissue extracts. Lanes 1 and 8: probe alone; lanes 2 and 9: probe plus nuclear extract from tumoral thyroid tissue; lane 3: probe plus nuclear extract from tumoral thyroid tissue in the presence of a 200-fold molar excess of unlabeled AP2 consensus oligonucleotide; lane 4: probe plus nuclear extract from tumoral thyroid tissue in the presence of a 200-fold molar excess of unlabeled AP2 mutant oligonucleotide, lane 5: probe plus nuclear extract from tumoral thyroid tissue in the presence of a 200-fold molar excess of unlabeled Sp1 consensus oligonucleotide; lane 6: probe plus nuclear extract from tumoral thyroid tissue in the presence of a 200-fold molar excess of unlabeled Sp1 mutant oligonucleotide; lane 7: probe plus nuclear extract from tumoral thyroid tissue in the presence of a 200-fold molar excess of unlabeled AP2 and Sp1 consensus oligonucleotides. In lanes 10 and 11 the probe was incubated with nuclear extract from tumoral thyroid tissue were incubated in the presence of a specific anti-AP2 (lane 10) or anti-Sp1 (lane 11) antibody, respectively, thus inducing a supershift of the complex. Arrows show the position of free (DNA) and bound (DNA-P) probes.

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