Figure 3

Nuclear β-catenin in the cerebellum and cortex of transgenic mice (A) While strong β-catenin immunoreactivity is present in the cellular processes making up the cerebellar glomeruli (arrow), no β-catenin is detected immunohistochemically in the blue hematoxilin-stained nuclei of internal granule layer (IGL) neurons of non-transgenic animals. (B) Many IGL neuronal nuclei contain β-catenin in Mut-1 transgenic animals. Scattered molecular layer neurons also have nuclear β-catenin (arrow), but Purkinje cell nuclei are negative (arrowhead). (C) In non-transgenic animals cerebral cortical neurons and glia do not contain nuclear β-catenin. (D) Many small to medium sized cortical neuronal nuclei are immunopositive for β-catenin (arrows). Original magnification for all images 600X.