Figure 2

β-catenin transgenic protein expression (A) A Western blot of total protein extracts from mouse cerebella probed with anti-FLAG antisera shows high levels of transgenic protein expression in one wild-type human β-catenin line (WT-1) and two stabilized mutant β-catenin lines (Mut-1, Mut-2). Lower expression is detected in the WT-2 wild-type and Mut-3 mutant lines. No expression is seen in the non-transgenic (NT) negative control lane. (B) A Western blot of mouse cerebellar protein divided into cytoplasmic (C) and nuclear (N) fractions and probed with an antibody recognizing endogenous murine as well as transgenic human β-catenin. Extracts from the high-expressing mutant lines Mut-1 and Mut-2 show elevated levels of cytoplasmic and nuclear protein in comparison to non-transgenic siblings. Antibody recognizing the cytoplasmic anti-heat shock protein 90 KDa (HSP90) controls for fractionation and loading.