Figure 3

The methylation profiles of the p14 ^ARF and p15 ^INK4b genes in the hepatoma cell lines analyzed by both MSP and Southern analyses. Panel 1, The schematic illustration of the gene structure and the CpG islands of interests along with restriction digestion sites and the probes. The scale is arbitrary. Panel 2, The methylation profiling of the p14^ARF and p15^INK4b promoter CpG island by MSP. Panel 3, The schematic illustration of the CpG islands of p14^ARF with restriction digestion sites and the probes. Panel 4, The electrophoretic pattern of the digests before blotting of the genomic DNA. The cell origins of the genomic DNA are indicated at the top. The size markers are provided by Hind III digests of the lambda phage DNA and indicated in kb at the left side of the autorad. Abbreviations: B, BstX I; H: Hpa II, M: Msp I. Panel 5, The autorad of the Southern analysis hybridized by the probe indicated in panel 3. The size of the hybridized band is indicated at the left side of the antorad. Panel 6, The schematic illustration of the CpG islands of p14^ARF with restriction digestion sites and the probes. Panel 7, The electrophoretic pattern of the digests before blotting of the genomic DNA. The cell origins of the genomic DNA are indicated at the top. The size markers are provided by Hind III digests of the lambda phage DNA and indicated in kb at the left side of the autorad. Abbreviations: Hi: Hind III; H: Hpa II, M: Msp I. Panel 8, The autorad of the Southern analysis hybridized by the probe indicated in panel 6.