Figure 5
From: Role of Bax in resveratrol-induced apoptosis of colorectal carcinoma cells
Representative colony formation assay with HCT116 and HCT116 bax -/- cells. When 1.5 × 103 live cells, counted in a CASY cell counter, were seeded into 25 cm2 tissue culture flasks and were incubated for 10 days in the absence of drug, bax -/- cells produced more colonies (left figure), indicating the survival advantage per se of bax-deficiency. Right figure: 7.5 × 103 live HCT116 cells and 1.5 × 103 bax -/- cells were seeded into flasks and exposed to the solvent DMSO (mock) or resveratrol dissolved in DMSO, 24 h later. Colonies were stained with crystal violet.