Figure 4From: Androgen and retinoic acid interaction in LNCaP cells, effects on cell proliferation and expression of retinoic acid receptors and epidermal growth factor receptorGel electrophoretic mobility shift assay of the interaction between androgen receptor (AR) and nucleotide sequences of the promoter of RAR α gene. Panel A, 1: 32P-probe (P) alone; 2: P + BSA (10 μg); 3: P + purified AR (3 μl, 3–5 ng/μl); 4: P + AR + 500 x non-radioactive probe and 5: P + AR + 500 x non-specific oligo probe. Panel B, 1: 32P-probe (P) + 10 μg nuclear extract (NE); 2–4: P + 10 μg NE + 100x, 300x and 500x non-radioactive probe, respectively. Panel C, 1: 32P-probe (P) + AR alone; 2: P + AR + 300 x non-radioactive probe; 3: P + AR + anti-AR.Back to article page