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Figure 4 | BMC Cancer

Figure 4

From: Androgen and retinoic acid interaction in LNCaP cells, effects on cell proliferation and expression of retinoic acid receptors and epidermal growth factor receptor

Figure 4

Gel electrophoretic mobility shift assay of the interaction between androgen receptor (AR) and nucleotide sequences of the promoter of RAR α gene. Panel A, 1: 32P-probe (P) alone; 2: P + BSA (10 μg); 3: P + purified AR (3 μl, 3–5 ng/μl); 4: P + AR + 500 x non-radioactive probe and 5: P + AR + 500 x non-specific oligo probe. Panel B, 1: 32P-probe (P) + 10 μg nuclear extract (NE); 2–4: P + 10 μg NE + 100x, 300x and 500x non-radioactive probe, respectively. Panel C, 1: 32P-probe (P) + AR alone; 2: P + AR + 300 x non-radioactive probe; 3: P + AR + anti-AR.

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