Figure 3
From: Optimisation of the RT-PCR detection of immunomagnetically enriched carcinoma cells
Expression of seven RT-PCR markers in 5 separate single MCF-7 cells lysed with 100 normal MNC. Lane M – pUC19/HpaII molecular weight marker. The following 3 lanes are amplifications from 100 PBMNC only. The following 5 lanes are amplifications from one MCF-7 cell in 100 PBMNC, Lane C is the cDNA positive control, Lane G is the genomic DNA control (G), Lane R is the RT – negative control; Lanes labelled N are the PCR negative controls. Genomic bands can be seen for many ELF3 amplifications,