Table 1 Oligonucleotides’ sequences and experimental conditions used for RT-PCR and EMSA analysis.
Experiment | molecule | Size (bp) | Sequence | # of Cycles | Hybridization Temp (°C) |
|---|---|---|---|---|---|
RT-PCR | MMP-9 | 409 | sense: 5’ CGCAGACATCGTCATCCAGT 3’ antisense: 5’ GGATTGGCCTTGGAAGATGA 3’ | 30 | 62 |
Ribosomal phosphoprotein | 486 | sense: 5’ GTTCACCAAGGAGGACCTCA 3’ antisense: 5’ CACATTAGGCAGAGGTGTCT 3’ | 25 | 50 | |
EMSA | NF-κB probe | 22 | sense: AGTTGAGGGGACTTTCCCAGGC antisense: GCCTGGGAAAGTCCCCTCAACT | - | 37 |
Mutant probe | 22 | sense: AGTTGAGGCGACTTTCCCAGGC antisense: GCCTGGGAAAGTCGCCTCAACT | - | 37 |