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Table 4 Relative normalized quantification of TKTL1 gene expression with the three published primer pairs TKTL1(1), TKTL1(2) and TKTL1(3)

From: TKTL1 expression in human malign and benign cell lines

Cells

TKTL1(1)

TKTL1(2)

TKTL1(3)

 

Mean ± SD

Cq

Mean ± Stabw

Cq

Mean ± Stabw

Cq

HTC116

0.071 ± 0.063

31.79

7.4x10−4 ± 8.0x10−5

30.46

3.4x10−3 ± 9.4x10−4

28.86

HEK 293-TKTL1

0.014 ± 3.1x10−4

34.66

48.9 ± 1.14

17.68

48.9 ± 0.80

17.26

HEK 293-control

0.117 ± 0.0046 **)

31.63

28.4x10−4 ± 2.5x10−4 **)

28.70

0.010 ± 0.001 **)

27.83

HeLa

0.379 ± 0.041

28.40

5.0x10−5 ± 2.0x10−5

33.13

0.012 ± 0.004

26.04

HepG2

1.3x10−3 ± 9.2x10−4

36.75

2.0x10−5 ± 3.0x10−5

35.03

1.1x10−4 ± 2.0x10−5

33.02

HT-29

2.5x10−3 ± 1.6x10−3

35.36

2.0x10−5 ± 2.0x10−5

33.83

4.0x10−5 ± 3.0x10−5

33.84

HUVEC

0.194 ± 0.05

32.23

2.4x10−4 ± 2.3x10−4

33.72

1.8x10−4 ± 3.0x10−5

34.99

JAR

0.462 ± 0.09

25.64

0.037 ± 0.0031

25.15

0.028 ± 8.30x10−4

24.08

JEG

0.039 ± 0.006

30.12

1.6x10−4 ± 4.0x10−5

30.90

3.6x10−4 ± 3.0x10−5

29.58

23132/87

9.9x10−3 ± 0.014

34.13

9.0x10−5 ± 9.0x10−5

32.81

1.6x10−4 ± 1.0x10−5

32.79

MCF-7

2.4x10−4 ± 0.002

34.76

1.0x10−5 ± 1.0x10−5

36.85

1.0x10−5 ± 1.0x10−5

35.64

MDA-MB 231

3.5x10−4 ± 3.3x10−4

37.34

1.0x10−5 ± 1.0x10−5

33.87

5.0x10−5 ± 1.0x10−5

32.79

Mel2a

0.161 ± 0.012

30.81

1.6x10−3 ± 3.4x10−4

28.52

4.4x10−3 ± 2.3x10−4

28.69

OVCAR

0.333 ± 0.073

28.71

5.0x10−5 ± 6.0x10−5

33.51

1.7x10−4 ± 5.0x10−5

32.33

PA1

0.021 ± 0.007

33.36

5.1x10−4 ± 1.0x10−4

30.83

1.3x10−3 ± 1.4x10−4

30.06

SiHa

0.023 ± 0.020

32.33

1.0x10−5 ± 1.0x10−5

35.57

4.0x10−5 ± 4.0x10−5

34.18

SKOV3

0.084 ± 0.062

31.16

5.1x10−4 ± 1.4x10−4

30.43

1.2x10−3 ± 3.6x10−4

29.99

U251

1.000 ± 0.117

22.84

0.046 ± 0.0015

23.16

0.036 ± 9.7x10−4

22.06

U87

1.4x10−3 ± 2.8x10−4

36.81

1.0x10−5 ± 1.0x10−5

35.97

7.0x10−5 ± 1.0x10−4

33.84

WiDr

3.6x10−3 ± 2.9x10−4

34.95

2.0x10−5 ± 3.0x10−5

34.14

8.0x10−5 ± 2.0x10−4

33.13

WS1

0.010 ± 0.007

35.56

1.0x10−5 ± 1.0x10−5

38.50

6.0x10−5 ± 4.0x10−5

35.59

  1. For the quantification of TKTL1 gene expression, actin and PPIA were used as endogenous controls. In addition to values of relative normalized expression the cycle of quantification (Cq) for each PCR amplicon is indicated (Cq presents the cycle number at which the amount of amplified PCR amplicon reaches the threshold level). Cq values higher than the cut-off of 35 was not considered as a reliable Cq expression value. It is obvious, that the relative expression levels of TKTL1 in most tested malign and benign cell lines are decreased in comparison to JAR and U251. For HEK293-TKTL1 transfectants, the PCR amplicons amplified with primer pairs 2 and 3 were successfully proofed for TKTL1 sequence, whereas for HEK293-control transfectants, amplicons amplified with the three primer pairs were without TKTL1 sequence (data not shown).