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Table 3 Western blot data obtained with the three anti-TKTL1 antibodies JFC12T10 (monoclonal), 1C10 (monoclonal) and Sigma Prestige (polyclonal)

From: TKTL1 expression in human malign and benign cell lines

Cell line

Antibody

 

JFC12T10

1C10

Sigma Prestige

 

Most prominent signal (kDa)

2nd prominent signal (kDa)

Further signals (kDa)

Most prominent signal (kDa)

Further signals (kDa)*

Most prominent signal (kDa)

Fibroblasts

-

-

27/40/55/60

-

-

-

HCT116

27

-

multiple

-

37/55/65

-

HEK 293 -control

27

37

45/55/65/70

-

-

-

HEK 293-TKTL1

65

27/37

multiple

65

-

65

HeLa

27

-

multiple

-

37/40/47/65

-

HepG2

27

37/45

multiple

-

-

-

HT-29

27

-

-

-

-

-

HUVEC

27

37/40/45/50

multiple

-

-

-

JAR

27

37

multiple

-

37/55

-

JEG

27

40

multiple

-

-

-

23132/87

27

37/45

multiple

-

-

-

MCF-7

27

-

multiple

-

37/40/50/70

-

MDA-MB 231

27

40

multiple

-

37/40

-

Mel2A

27

45/40

multiple

-

45/55/70

-

OVCAR

27

42

multiple

-

37/40/50/90

-

PA1

27

37

multiple

-

55

-

SiHa

27

42

multiple

-

-

-

SKOV3

27

37

multiple

-

37740/55

-

U251

27

40/45/55

multiple

-

45

-

U87

27

55

multiple

-

45

-

WiDr

27

42

multiple

-

-

-

WS1

-

-

40/55/70*

 

40/55/65

-

  1. Western blot staining results with the antibodies JFC12T10, 1C10 and Sigma Prestige of at least two independent blots per cell line per antibody. Legend: “-“ no staining (no signal); “*” very faint staining. The polyclonal Sigma Prestige antibody did not show any signals despite the positive control.