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Table 2 Immunohistochemical data

From: TKTL1 expression in human malign and benign cell lines

Cell line

Monoclonal anti-TKTL1 antibody JFC12T10

Polyclonal anti-TKTL1 antibody Sigma Prestige

 

Nucleus

Cytoplasm

Cell surface

Nucleus

Cytoplasm

Cell surface

control:

      

HEK 293-control

-

++

-

-

-

-

HEK 293-TKTL1

-

+++

-

+

+++

-

benign:

      

Fibroblasts

-

++

-

-

[+]

-

HUVEC

-

+

++

-

[+]

-

WS1

-

+

++

-

-

-

malign:

      

HCT116

-

++

-

-

[+]

-

HeLa

-

+++

-

-

-

-

HepG2

[+]

++

-

+

-

-

HT-29

-

-

-

-

-

-

JAR

-

++

-

-

[+]

-

JEG

-

++

-

-

[+]

-

23132/87

-

+

-

-

-

-

MCF-7

[+]

-

-

-

[+]

-

MDA-MB 231

-

++

-

-

-

-

Mel2A

-

++

-

-

[+]

-

OVCAR

+

++

-

-

-

-

PA1

-

+

-

-

+

++

SiHa

-

++

-

-

[+]

+

SKOV3

-

++

-

-

[+]

-

U251

-

+

-

-

-

-

U87

+

+++

-

-

[+]

-

WiDr

-

+++

-

-

-

-

  1. The semiquantitative staining pattern is given for both antibodies (JFC12T10 and Sigma prestige) based on the analysis of two independent staining results per cell line per antibody. Legend: “-“: negative; “[+]”: faint positive; “+”: positive; “++”: strong positive; “+++” very strong positive.