125I seed irradiation is associated with greaterin vivoanticancer activity than X-ray. (A) Representative images of tumors treated with 125I seed and X-ray irradiation. (B, C) tumor weight and tumor size were measured after irradiation. Data are presented as mean ± SD (n =3). Significant differences between the 125I seed and X-ray groups under the same dose are indicated by *P < 0.05 and **P < 0.01. (D, E) Proteins with the involvement of cell growth and epithelial–mesenchymal transition were further detected by IHC and western blotting.