Figure 2

Apoptosis and G2/M arrest in glioblastoma multiforme (GBM) cells is induced by¹²⁵I seed irradiation. Apoptosis of GBM cells was examined by Annexin V-PI co-staining flow cytometric analysis (A, B) and caspase-3 activity assay (C). (D) Effects of ¹²⁵I seed on the cell-cycle distribution of GBM cells were examined by flow cytometric analysis. (E) TUNEL assay was used to evaluate the apoptotic response in U251 (left panel) and U87 (right panel) cells. (F) Treatment of cells with ¹²⁵I seeds caused obvious apoptosis in a dose-dependent manner, accompanied by up-regulation of cleaved caspase-3, cleaved PARP, and phosphorylated Cdc2 (Tyr 15). Data are presented as mean ± SD (n =3). Significant differences between the ¹²⁵I seed and X-ray groups under the same dose are indicated by *P <0.05 and **P < 0.01.