DZNep and SAHA effects on prostate cancer cell lines. Total RNAs were isolated from PC3, DU145 and LNCaP cells treated with 2 μM SAHA and 10 μM DZNep for 72 h. Results were analyzed by RT-qPCR. Relative changes in gene expressions compared to the control (the value of control was designed as 1) were calculated using comparative ΔΔCt method. The 18S RNA was used for normalization. Value = means ± SD from at least three measures, *p < 0.05.