Figure 1

Identification of miR-1290 target sites in the NAT1 3′-UTR. A, Schematic of the 3′-UTR-containing reporter constructs for potential miR-1290 target sites in NAT1. The 3′-UTR of the NAT1 gene was inserted just downstream of the firefly luciferase gene in the pMIR-report luciferase plasmid (NAT1-wt). Next, the mutated derivatives (Nat1-mut1, -mut2 and –mut1 + 2) of NAT1-wt were generated by inserting mutations into two putative binding sites corresponding to the seed-sequence of miR-1290. B-E, Cells were transfected with either miR-1290 or nonspecific control miRNA (NC). Luciferase activity was assayed 24 hr later. The data are shown as luciferase activity relative to the vehicle (pGL4.74).