Downregulation of IL-6 in tumor-associated endothelial cells inhibits tumor growth.
A, ELISA for IL-6 expression in HDMEC transfected with shRNA-IL-6 or with a control scrambled oligonucleotide sequence (shRNA-C). B, xenograft human tumors were generated in SCID mice by co-implanting HeLa and HDMEC-shRNA-IL-6 or control HDMEC-shRNA-C. Tumors growth was analyzed with calipers every 2 days for the duration of the experiment. C, macroscopic view of two representative xenograft tumors per group. D-E, graphs depicting tumor volume (
) and tumor weight (
) after retrieval (28 days post-implantation). Asterisk depicts p < 0.05.