Figure 6

Reversibility of DZNep-induced KRT13 expression in OSCC cells. (A) Schematic of DZNep treatment time. The inverted triangle denotes the starting point. White and black boxes represent incubation without and with 10 μM DZNep, respectively. SAS cells were incubated without DZNep for 72 h (control), with DZNep for 72 h (DZNep (+)), or with DZNep for 24 h followed by the removal of DZNep (DZNep (+/-)). KRT13 mRNA levels were analyzed by quantitative RT-PCR. Fold change in KRT13 mRNA was normalized to GAPDH and calculated relative to that of the cells under the control condition. The means ± SEM for each group (n = 3) are shown. Statistical analysis was performed by a one-way analysis of variance followed by Dunnett’s post hoc test. **p < 0.01, N.S. (not significant). (B) Ezh2 and Suz12 protein levels were examined by western blotting. Representative images are shown. Fold change in Ezh2 and Suz12 proteins was normalized to β-actin and calculated relative to band intensity of the control condition samples. The means ± SEM for each group (n = 4) are shown. Statistical analysis was performed by a one-way analysis of variance followed by Dunnett’s post hoc test. **p < 0.01.