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Figure 6 | BMC Cancer

Figure 6

From: SC-III3, a novel scopoletin derivative, induces cytotoxicity in hepatocellular cancer cells through oxidative DNA damage and ataxia telangiectasia-mutated nuclear protein kinase activation

Figure 6

Effect of SC-III3 on DNA damage and ATM/ATR pathways. (A) HepG2 cells were treated with SC-III3 for 24 hours. Western blot analysis of p-H2AX, total H2AX, p-Chk1 (Ser280) and p-Chk1 (Ser296) response to SC-III3. (B) HepG2 cells were treated with SC-III3 for 24 hours. Western blot analyses of the ATM/ATR signaling related proteins in HepG2 cells. (C) HepG2 cells were treated with 1 μM SC-III3 for different times as indicated. Western blot analyses of p-ATM, p-ATR, p-Chk1, p-Chk2, Cdc25C, p-CDK2, p53, and p21 in HepG2 cells. These experiments were done in triplicates.

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