SC-III3 induced HepG2 apoptosis in HepG2 cells. (A, B) Cells were treated with SC-III3 for 24 h. Annexin V/PI double-staining assay of HepG2 was analyzed by flow cytometry. The percentages of cells in apoptosis were represented. (C, D) Cells were treated with SC-III3 for 48 h. (E) HepG2 cells were treated with SC-III3 for 48 h. The protein levels of Bcl-2 and Bax were analyzed with western blotting. Data were presented as means ± SEM of three separate experiments. The difference were significant at *p < 0.05, **p < 0.01 vs. control.