G469V mutations over both space and time. Raindance Raindrop digital PCR was used to confirm KRAS and BRAF mutation status in all 8 tumor samples and the corresponding normal, with the four samples from the primary surgery (Sample 10P1-10P4) shown on the left and a representative sample from the recurrent surgery (Sample 10R1) and the corresponding normal (Sample 10 N) shown on the right. The relative location of each sample in the patient is shown in the bottom right, with those from the primary surgery colored in green and those from the recurrent surgery colored in orange (courtesy of Vicky Earle, UBC graphics). Similar to Figure 3, the wild type ('WT’) and mutant ('MUT’) population are circled in each panel, with the % MUT indicated in the top right corner (MUT drops/total of WT + MUT droplets). The KRAS G12V mutation was detected in Samples 10P1 and 10P2, while the BRAF G469V mutation was exclusively detected in Sample 10P3. All remaining samples were negative for KRAS G12V, BRAF G469V and NRAS Q61R (not shown).