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Figure 4 | BMC Cancer

Figure 4

From: Regulation of Mcl-1 by constitutive activation of NF-kappaB contributes to cell viability in human esophageal squamous cell carcinoma cells

Figure 4

Attenuation of Mcl-1 expression by NF-κB inhibitor or dominant negative mutant of IκBα in various human esophageal squamous cell carcinoma cell lines. (A, B) Inhibition of NF-κB pathway by NF-κB-specific inhibitor Bay11-7082 prevented Mcl-1 expression in TE-1 (A) and KYSE150 (B) cell lines. TE-1 or KYSE150 cells were treated with indicated concentrations of DMSO or Bay11-7082 for 24 h, whole cell lysates were harvested. Mcl-1 was determined by Western blotting. GAPDH was used as a loading control. Data shown are representative of at least two independent experiments. Statistical significance: * p < 0.05 and **p < 0.01, compared with the DMSO control. (C, D) Expression of dominant negative mutant of IκBα (DNMIκBα) decreased Mcl-1 protein level in TE-1 (C) and KYSE150 (D) cell lines. TE-1 or KYSE150 cells seeded in 12-well plate were untransfected (Mock), transfected with 1600 ng/well pcDNA3.1 empty vector or 1600 ng/well DNMIκBα expression plasmid using Lipofectamine™ 2000 according to manufacturer’s instructions. Cells were harvested at 24 h after transfection and subjected to Western blotting analysis with anti-Mcl-1 antibody. GAPDH was used as a loading control. Data shown are representative of at least two independent experiments. Statistical significance: **p < 0.01, compared with the pcDNA3.1 empty vector-transfected control.

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