Expression of Mcl-1 protein and mRNA in human esophageal squamous cell carcinoma cell lines. (A) Expression of Mcl-1 protein in various esophageal carcinoma cell lines. Whole cell lysates of TE-1, Eca109, KYSE150 and KYSE510 as well as HaCaT cell lines were subjected to immunoblotting analysis with anti-Mcl-1 antibody. GAPDH was used as a loading control. (B) Total RNA was isolated from above-mentioned cell lines and subjected to RT-PCR, using specific primers designed to amplify Mcl-1 and GAPDH mRNAs. GAPDH was used as a loading control. (C)
Mcl-1 mRNA expression levels of TE-1, Eca109, KYSE150 and KYSE510 as well as HaCaT cell lines were analyzed by quantitative real-time RT-PCR. The Mcl-1 mRNA level of HaCaT cells was normalized to a value of 1. Fold-change in mRNA levels was shown. Data are presented as the mean ± S.D. of two independent experiments performed in triplicate. Statistical significance: *, p < 0.01, compared with HaCaT cells.