Figure 5

Immunohistochemical analysis of SIRT1 expression in primary hepatocellular carcinoma (HCC) surgical specimens. (A) and (B) Nuclear expression of SIRT1 was negative in 23/99 of HCC tissues (A), and positive in 76/99 (B) (400 × magnification in A–B). (C) The patients with HCC were divided into positive- and negative-expression groups based on SIRT1 immunostaining scores. The survival rate of the patients in the positive-expression group was significantly lower than that of patients in the negative-expression group (P = 0.035 by the log-rank test).