SIRT1 induces epithelial-mesenchymal transition (EMT) in hepatocellular carcinoma (HCC) cells. (A) - (D) RT-PCR was used to detect changes in the expression of EMT-associated genes in HCC cells treated with nicotinamide (NAM) or resveratrol (RES). Results represent means of triplicate experiments ± standard errors (*P < 0.05). (E) Immunofluorescence staining for E-cadherin and vimentin was performed in HepG2 and SMMC-7721 cells that were either untreated or treated with NAM or RES. Nuclei were counterstained with DAPI (×200).