Figure 4

E2F5 was involved in miR-181a inducing promotion of HCC cells proliferation. (A) Western blot was performed to analyze E2F5 expression in siE2F5 transfected SMMC-7721 cells. β-actin was used as an internal quantitative control. (B) Effect of siE2F5 on cell proliferation was measured by MTS assay. (C) SMMC-7721 cells were transfected with NC, miR-181a inhibitor or miR-181a inhibitor and siE2F5, and then MTS assay was performed. (D) Representative pictures of colony formation assay of siE2F5 transfected cells. Colonies were evaluated and values were reported as the ratio between siE2F5 transfected cells and NC transfected cells. *P < 0.05.