Anti-CD40 enhances local but not systemic CTL responses induced by IMQ. BALB/c mice bearing AB1-HA tumors were left untreated or partially (75%) surgically debulked of tumor then treated with immunotherapy (IMQ; 50 μg i.t. q1dx6 on day of surgery and/or anti-CD40; 100 μg i.p. q1dx1, starting one day after surgery). CFSE-labelled, CL4-pulsed and un-pulsed target cells were injected i.v. on day 25 post inoculation and 18 hours later the dLN, ndLN and spleen were harvested and analysed by flow cytometry to assess levels of CTL activity. ns = not significant, *p < 0.05, **p < 0.01 compared to untreated; Kruskal-Wallis test with Dunn’s correction for multiple comparisons.