DEK downregulation decreases cell viability, migration and invasion. A) Three different siRNAs of DEK (siDEKsec57, sec58 and sec59) were used to downregulate DEK protein expression in DLD1 and SW620 cell lines. We verified DEK expression levels by western blot at 24, 48, and 72 hours after transfection. B) MTS assay showed that both DLD1 and SW620 cell lines decreased cell viability at 72 hours after DEK downregulation (P < 0.001). C) Microscope images of wound healing assay showed a reduced cell migration after DEK downregulation in both cell lines. Images are representative from one experiment and were taken at 6, 12, and 24 hours after scratching. Arrows represent distance between cell migration heads. D) Cell invasion assays performed in Boyden chamber showed that DEK silencing decreased invasion ability of both cell lines. All assays were performed with 2 different siRNA sequences (siDEKsec57 and 58).