Figure 5

Wound-healing and matrigel invasion assay on MIA PaCa-2 and PANC-1 cells. Wound-healing assay on MIA PaCa-2 (panel A) or PANC-1 cells (panel E) treated or not with fMLP (50nM), Ac2-26 (1 μM), Boc-1 (10 μM), fMLP + Boc-1 or Ac2-26 + Boc-1; Statistical significance was calculated using two way ANOVA,* p < 0.05, **p < 0.01, ***p < 0.001 vs untreated control; ^##p < 0.01 and ^§§p < 0.01 vs respective controls. Wound-healing assay on MIA PaCa-2 (panel B) or PANC-1 cells (panel F) treated or not with ANXA1 blocking antibody (AbANXA1) or scrambled rabbit IgG (IgG R); Statistical significance was calculated using unpaired t-test between control and treated cells, ***p < 0.001 vs untreated control. All wound-healing data are representative of 5 experiments ± SEM. Invasiveness rate of MIA PaCa-2 (panel C) or PANC-1 cells (panel G) treated or not with fMLP (50 nM), Ac2-26 (1 μM), Boc-1 (10 μM), fMLP + Boc-1 or Ac2-26 + Boc-1; Statistical significance was calculated using two way ANOVA, ***p < 0.001 vs untreated control; ^###p < 0.001 and ^§§§p < 0.001 vs respective controls. Invasiveness rate of MIA PaCa-2 (panel D) or PANC-1 cells (panel H) treated or not with AbANXA1 or IgG R; Statistical significance was calculated using unpaired t-test between control and treated cells, ***p < 0.001 vs untreated control. Invasiveness rates were measured as described in Methods section. Data represent mean cell counts of 12 separate fields per well ± SEM of 5 independent experiments.