FPR-1 and 2 expression and activation in MIA PaCa-2 and PANC-1 cells. A, Cell surface expression of FPR-1 and FPR-2 in MIA PaCa-2 and PANC-1 cells was analyzed by flow cytometry. The violet areas in the plots are relative to secondary antibody alone. FPR-1 and FPR-2 signals are showed with green bends. B, Expression of FPR-1 and FPR-2 in MIA PaCa-2 and PANC-1 cells analyzed by PCR. Effects of fMLP (50 nM), Ac-2-26 (1 μM) and FPR pan-antagonist Boc-1 (10 μM) on the FPR-induced rise in intracellular Ca2+ in MIA PaCa-2 (panel C) or PANC-1 cells (panel D). Cells were treated as described in Methods section. The histograms show the fluorescence ratio calculated as F340/F380 nm in absence of extracellular Ca2+. Control represents ionomycin-stimulated cells. Data are means ± SEM (n = 5).