Expression and localization of cleaved and secreted form of ANXA1 from MIA PaCa-2 and PANC-1 cells. Cellular compartments were obtained as described in Methods section. Total (T), membrane (M), EDTA Wash (EW), cytosolic (C), nuclear (N) and supernatant (S) ANXA1 expression in protein extracts from MIA PaCa-2 and PANC-1 was examined by Western blot with anti-ANXA1 antibody. The protein bands were normalized on tubulin levels. The data are representative of 5 experiments with similar results.