Figure 6

AR binds to sites located between Exon 0 and Exon 1 of the L1CAM gene. (A), Schematic representation of the localization of the AR binding sites in the L1CAM gene. Upper row: Distal localization of the AR binding sites in relation to the L1CAM promoter region. Middle row: localization of binding sites 1 and 2 (BS 1+2) and binding site 3 (BS 3) between Exon 0 and Exon 1. Lower row: The localizations of primer products PP1 and PP2 are shown. An immune-precipitation (IP) of MDA436 cells transfected with AR-EGFP or mock was performed with an AR (AR-IP) antibody or a IgG (IgG-IP) control antibody. Precipitated DNA was analyzed by qPCR amplification for BS 1+2 (B) and BS 3 (C) (n = 3). The input was used as a positive control. Agarose gel electrophoresis of the BS 1+2 (D) and BS 3 (E) amplification products. (F) Precipitated DNA was analyzed by qPCR amplification for the AR binding site in the CAMKK gene. Note that the same AR-IP material was used but CAMKK specific primers (n = 3).