Figure 3

Western blot analysis of intracellular HMGB1 in MDA-MB-231 human breast cancer cells treated with fibroblast CMs (BCF 044 and NTF 044) for 6, 24, and 48 h. Cancer cells cultured in fresh medium were used as a negative control. The intensity of each HMGB1 band is shown after normalization against the β-actin internal loading control protein. Bar graphs represent mean ± SD of two independent experiments. * = p-value of less than 0.05 comparing HMGB1 levels in the CM-treated cells with controls at each time point; # = p-value of less than 0.05 comparing HMGB1 levels in BCF-CM-treated cells with NTF-CM treatment.