Figure 2

PRKDC gene suppression in MYC-overexpressing human lung fibroblast cells decreases cell viability. A) Gene and protein knockdown efficiency with independent shRNA clones against PRKDC quantified by RT-PCR and immunoblotting, respectively, in WI-38 cells. Protein was analyzed via immunoblotting for PRKDC (anti-PRKDC) and GAPDH (anti-GAPDH). B) Stable WI-38 cell lines were exposed to increasing amounts of lentivirus expressing PRKDC shRNAs and subjected to a cell viability assay after 6 days. C) Stable WI-38 cell lines were treated with varying concentrations of a PRKDC inhibitor, NU-7441, for 3 days and subjected to a cell viability assay. Data are shown as mean ± SD. Statistical analysis using one-way ANOVA; ****P ≤0.0001; ***P ≤ 0.001; **P ≤ 0.01.