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Figure 1 | BMC Cancer

Figure 1

From: Prevalence of HMTV in breast carcinomas and unaffected tissue from Mexican women

Figure 1

End-point PCRs for detection of the MMTV env g ene in the samples. A. First nested PCR round resulting in a 660-bp MMTV env gene fragment, exemplifying the expected results defining positive detection. B. Second nested PCR round resulting in a 250-bp fragment, exemplifying the expected results defining positive detection. C. Three samples that exemplify positive and negative detection of the 250-bp PCR product. MW: 100-bp molecular weight ladder; Neg: non-template negative control; C3H: strain with MMTV env gene inserted into pBR322 plasmid, used as a positive control; T: tumor; and NB: unaffected tissue.

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