Figure 5
From: Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib
Androgen inhibition impairs Sorafenib resistance in PTEC. (A): Analysis of Androgen receptor (AR) mRNA expression levels by qPCR in PTEC cell lines. Data were normalized to 18S rRNA and to 1 for HME. Data are mean ± S.E.M. of three different experiments. (B): AR and actin protein expression by Western blot in wild type HEK, HEK overexpressing AR (HEK-AR), LNCaP, HMEC and PTEC2 line. (C): Cell proliferation of PTEC (black columns) and HUVEC (white columns) after 48 h incubation with 1 μM Sunitinib or Sorafenib in the presence or absence of 10 μM Casodex. Data are the mean ± S.E.M. of a minimum of three independent experiments performed with two PTEC cell lines (PTEC2 and 3) in triplicate. Statistical significance *p < 0.05.