Growth and colony formation assays of MGC-803 cells following overexpression or knockdown of
LEIGC. Cells were transfected with LEIGC-shRNA vector (shRNA) and scrambled shRNA vector (NC1), or LEIGC vector (lncRNA) and non-related lncRNA vector (NC2), and blank control (blank). (A). Basal levels of LEIGC in MGC-803, SGC-7901 and AGS cells. (B). Knockdown and overexpression of LEIGC was confirmed by qRT-PCR. (C). Cell proliferation rate was determined by measuring the absorbance at 490 nm in MTS assays. (D and E). Colony formation assays of MGC 803 cells. *P>0.05.