Alterations in lncRNA expression profiles between gastric tumor tissues and paired adjacent non-tumorous tissues. (A). lncRNA expression was evaluated by an lncRNA expression microarray. Results from hierarchical clustering showed different lncRNA expression among samples. “Red” indicates high expression; “green” indicates low expression. (B). Results from qRT-PCR experiments demonstrating downregulated expression of lncRNAs in three gastric cancer samples. (C). qRT-PCR analysis of lncRNAs selected from microarray results in three gastric cancer samples revealed upregulated expression. (D). qRT-PCR verification of mRNA selected from microarray results in three gastric cancer samples. (E). Expression of lncRNA LEIGC in fresh gastric cancer tissues from 35 patients was detected by qRT-PCR. LEIGC levels were normalized to GAPDH and expressed in terms of the threshold cycle (CT) ratio. Error bars represent the means ± SEM. *P < 0.05; **P < 0.01.