Detection of cell cycle by flow cytometry. (A) Flow cytometry was used to examine BIU cell cycle. G1 / G2, S, G2/M phase occupied 57.19%, 13.14%, 16.72%, respectively. (B) & (C) With the increasing treatment time (24 h, 48 h) of PMA, the number of BIU cells increased gradually in S phase and G2/M phase, and S phase was 22.33% for 24 h, 36.41% for 48 h; G2/M phase was 23.39% for 24 h, 34.42% for 48 h. (D) & (E) With the increasing treatment time (24 h, 48 h) of calphostin C, the number of BIU cells decreased noticeably in S phase and G2/M phase, and S phase was 8.39% for 24 h, 4.92% for 48 h; G2/M phase was 10.55% for 24 h, 7.46% for 48 h. The data are representative of 3 individual experiments. (F) Summary of cell cycle distribution in panel A-E.