Figure 8

Proliferation and invasion ability of BIU were inhibited by calphostin C and promoted by PMA. (A) CCK-8 assay was used to examine BIU cell proliferation. cell proliferation was inhibited on dose-dependent correlation with the increasing concentration (1, 3, 5, 7 μmol/L) of calphostin C at 24 h, 48 h, 72 h. (B) Cell proliferation was promoted by PMA on correlation with the increasing concentration (50, 100, 200, 400 nmol/L) at 24 h, 48 h, 72 h. (C) According A450 of BIU at different times, cell proliferation curve was drawn. Compared with BIU, cells with calphostin C were inhibited and cells with PMA were promoted. (D) & (E) According the optical density value (A) of each well measured, BIU cell growth inhibition/activation ratio was calculated as (1 – A₄₅₀ of experimental well/A₄₅₀ of blank control well) × 100%, with different concentration of PMA (50, 100, 200, 400 nmol/L) and calphostin C (1, 3, 5, 7 μmol/L), we calculated the activation/inhibition ratio. Each data are representative of 3 individual experiments. IC50 of calphostin C =7.4 μmol/L, and IC50 of PMA = 24 nmol/L.