Figure 6

Expressions by RT-PCR and western blot in bladder cells with drugs. (A) & (B) Quantitative real-time PCR (RT-PCR) were used to evaluate netrin-1 & UNC5B’s expression in treatment of cells (BIU & SV) with drugs- PMA (PKC α agonist) and calphostin C (PKC α inhibitor). The results showed that the cells were significantly inhibited by calphostin C (24 h) in netrin-1’s expression and promoted by PMA (24 h), and cells were first promoted, and then inhibited by PMA (4 h) then calphostin C (24 h). Whlie UNC5B was opposite. (C) Confirmation of netrin-1 down-regulation with calphostin C and up-regulation with PMA by western blot. (D) The relative protein expression of netrin-1 & UNC5B in treatment of cells with drugs, GAPDH as an internal control (P < 0.05). *P < 0.05 compared with BC cell line BIU (paired t-test).