Figure 2

Expressions by RT-PCR and western blot in different stages and grades. (A) UNC5B expression was different in BC specimens with different clinical pathological stages (T1, T2, T3 & T4) at mRNA level. Data was analyzed by Student’s t test and shown as mean ± SD of ten independent experiments. UNC5B expression in stage T4 was much less than in T1–T3 stages (P < 0.05). *P < 0.05 compared with stage T1 BC tissues (paired t-test). (B) Different expressions of UNC5B in pT stage were detected by western blot and UNC5B expression in stage T4 was sharply less than in T1–T3 stages (P < 0.05). (C) UNC5B mRNA expression was determined by RT-PCR in different grades (G1, G2 & G3). Differences at G1 & G3 were significant. *P < 0.05 compared with G1 BC tissues (paired t-test). (D) Netrin-1 expression was different in BC specimens with different pT stage at mRNA level by RT-PCR. Stage T4 was more than in T1–T3 stages (P < 0.05). (E) Western blot was used to detect netrin-1 expression in different stages. The band intensities indicate netrin-1 expression more in stage T4. (F) Netrin-1 expression was determined by RT-PCR in different grades (G1, G2 & G3), and G3 was the highest (P < 0.05). *P < 0.05compared with G1 BC tissues (paired t-test). All data are representative of 3 individual experiments.