Expression by RT-PCR and western blot in BC tissues (T) and normal bladder tissues (N). (A) The average netrin-1 & UNC5B expression ± SD for all studied tumors (120 cases) and their corresponding normal tissues (40 cases) by RT-PCR, bar graphs describe significant UNC5B down-regulation and netrin-1 up-regulation in T in comparison with N (P < 0.05), β-actin as an internal control. (B) UNC5B protein was detected by western blot in 40 pair tissues and band intensities indicate UNC5B expression conspicuously lower in T comparing with N. GAPDH was used as a loading control. (C) The ratio between the optical densities of UNC5B & GAPDH in the same tissue was calculated and expressed graphically. Significant differences of UNC5B expression between T & N were analyzed statistically and UNC5B expression was obviously greater in N (P < 0.05). (D) Netrin-1 protein was detected by western blot and increased in T. (E) The relative protein expression of netrin-1, GAPDH as an internal control (P < 0.05).