Figure 6
From: Amyloid-β precursor protein promotes cell proliferation and motility of advanced breast cancer
APP promotes cell migration of MDA-MB-231 and its expression is elevated in invasive breast cancer of human tissues. (A) The cell motility of APP knockdown (shAPP) MDA-MB-231 was examined in wound healing assay. Following the wounding, cells were untreated or treated with IGF-1 (25 ng/ml) for 18 hours in 0.1% serum containing medium. Cells were then fixed and stained for clear demonstration (scale bar = 200 μm). (B) The role of APP for cell migration was evaluated in Boyden chamber assay in serum-free medium with or without IGF-1 (50 ng/ml) for 18 hours. The rectangular area was further magnified for demonstration of different cell morphology. (C) The migrated cells in panel B were counted in three randomly selected areas. (D) No staining for APP (22C11) is present in this normal terminal duct lobular unit. (E) The well-differentiated grade 1 invasive ductal carcinoma shows weak staining for APP. (F) The poorly-differentiated grade 3 invasive ductal carcinoma shows strong staining for APP. Scale bar = 100 μm.