APP modulates breast cancer cell growth in 3D culture and in xenografted model. MDA-MB-231 cells were subjected to 3D Matrigel on-top assay. The cells were seeded (2x104/well) in 48-well plate coated with Matrigel in triplicate and then cultured for 7 days with medium change in every two days. The morphology of growing cells were obtained (A) and followed by MTT assay (B). (C) The control and shAPP-7 MDA-MB-231 (2x106) cells were injected into nude mice s.c. (n = 6) and allowed to grow for 6 weeks. The grown tumors were excised and the grown tumor size compared. (Scale bar = 1cm) (D) The independent xenograft study (2.5x105 cells s.c injected; n = 5, respectively) revealed that shAPP-7 MDA-MB-231 cell growth rate was largely decreased as compared to control group (p < 0.01).