Figure 5

Microglial cells are recruited from the mouse brain parenchyma. A, in the contralateral hemisphere we noted ramified microglia (IB4, magenta; DAPI in cyan), whereas in the core of the tumor mass (B, DAPI in cyan) we observed hVim⁺ tumor cells (C, orange). D, In contact with GBM (hVim⁺ cells, orange), infiltrated microglia exhibit amoeboid morphology (IB4, magenta). GBM95 cells were injected in the striatum of immunecompetent mice 14 days before the immunohistochemical analysis. Data represent four separate experiments. Scale bars, 40 μm. hVim = human vimentin. Cc = corpus callosum; LV = lateral ventricle; Str = striatum.