Human GBM is highly angiogenic. (A
B) CD31 immunostaining (red) shows blood vessels displaying a regular morphology in the contralateral hemisphere. (C
D) Disrupted wall of an irregular blood vessel (CD31+) in the injected hemisphere demonstrates a chaotic angiogenesis. Cell nuclei counterstaining by DAPI (cyan). (C’) Macroscopic view of freshly dissected brain reveals the presence of irregular blood vessels (arrowheads) and necrotic area (asterisk). Data represent four separate experiments. Scale bar, 40 μm.cc = corpus callosum; lv = lateral ventricle; Str = striatum.