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Figure 2 | BMC Cancer

Figure 2

From: The orthotopic xenotransplant of human glioblastoma successfully recapitulates glioblastoma-microenvironment interactions in a non-immunosuppressed mouse model

Figure 2

Histopathological and immunohistochemical characteristics of the tumor mass within mouse brain parenchyma 14 days after cell implantation, and of the original patient’s biopsy. A, Neoplastic cells forming a circumscribed solid tumor mass into brain tissue (black asterisk); the blue asterisk indicates a necrosis area in the core of the tumor mass. B, Neoplastic cells showing prominent anaplasia and mitotic figures (arrow > inset). C, Glomeruloid vessels (*). D, Human astrocytes inoculated in mouse brain; no tumoral mass is formed. Data are representative of four separate experiments. E, Microscopic analysis of the patient’s original biopsy showing anaplastic cells (*, top right) and tumoral necrosis (bottom left). F and G, Negative immunostaining for IDH1–R132H mutation in both tumor mass within mouse brain parenchyma (F) and in patient’s biopsy material (G). H, Grade II oligodendroglioma, a positive control case of the IDH1–R132H mutation. Scale bars, 100 μm (A); 50 μm (B, E, H); 80 μm (C, D); 30 μm (F, G).

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