Figure 4

NA induces PLC, PKC and ERK activation in PC3 cells. (A) The measurement of IP₃ levels using the IP₃ [³H] Radioreceptor assay kit was performed in PC3 cells vehicle-treated (control) or treated for different times with NA (100 μM). Data shown are the mean ± SD of three separate experiments. Statistical analysis was performed by comparing NA-treated with control cells, *p ≤ 0.01. (B-C) Lysates from PC3 cells vehicle-treated (control) or treated for different times with NA (100 μM) were separated on SDS-polyacrylamide gels, transferred and then blotted with anti-pERK, anti-ERK, anti-p38, anti-pp38 and anti-phospho-(Ser) PKC substrate Abs. The GAPDH protein level was evaluated as a loading control. Data shown are representative of one out of three separate experiments. Densitometric analysis was performed evaluating three different experiments and statistical analysis was carried out comparing NA-treated with control cells, *p ≤ 0.01.